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验证数据展示

  • WB analysis using 21069-1-AP (same clone as 21069-1-PBS)

    WB analysis using 21069-1-AP (same clone as 21069-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

    IP experiment of mouse skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

    IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 21069-1-AP (same clone as 21069-1-PBS)

    IHC staining of mouse testis using 21069-1-AP (same clone as 21069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21069-1-AP (SIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

SIM2 Polyclonal antibody, PBS Only

SIM2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 21069-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

Class E basic helix-loop-helix protein 15, SIM, Single minded homolog 2, Single-minded homolog 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21069-1-PBS targets SIM2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15204

Product name: Recombinant human SIM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 342-525 aa of BC110444

Sequence: LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 single-minded homolog 2 (Drosophila)
别名 Class E basic helix-loop-helix protein 15, SIM, Single minded homolog 2, Single-minded homolog 2
计算分子量 667 aa, 73 kDa
观测分子量 60-64 kDa
GenBank蛋白编号BC110444
基因名称 SIM2
Gene ID (NCBI) 6493
RRIDAB_10732844
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14190
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

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