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SIM2 Polyclonal antibody

SIM2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21069-1-AP

Print datasheet

Synonyms

bHLHe15, SIM, SIM2, Single minded homolog 2

验证数据展示

  • WB analysis using 21069-1-AP

    WB analysis using 21069-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 21069-1-AP

    IP experiment of mouse skeletal muscle using 21069-1-AP

    IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.

经过测试的应用

Positive WB detected inCOLO 320 cells, human skeletal muscle tissue, HeLa cells, HepG2 cells, U-251 cells
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

ChIPSee 1 publications below

产品信息

21069-1-AP targets SIM2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsChIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SIM2 fusion protein Ag15204 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name single-minded homolog 2 (Drosophila)
Synonyms bHLHe15, SIM, SIM2, Single minded homolog 2
Calculated Molecular Weight 667 aa, 73 kDa
Observed Molecular Weight 60-64 kDa
GenBank Accession NumberBC110444
Gene Symbol SIM2
Gene ID (NCBI) 6493
RRIDAB_10732844
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14190
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

实验方案

Product Specific Protocols
WB protocol for SIM2 antibody 21069-1-APDownload protocol
IP protocol for SIM2 antibody 21069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanChIP

Biochem J

Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes.

Authors - Emily Lyn Button
View Article