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验证数据展示

  • WB analysis using 21069-1-AP

    WB analysis using 21069-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 21069-1-AP

    IP experiment of mouse skeletal muscle using 21069-1-AP

    IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.

SIM2 Polyclonal antibody

SIM2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 21069-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA and More (1)

别名

bHLHe15, SIM, SIM2, Single minded homolog 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, human skeletal muscle tissue, HeLa cells, HepG2 cells, U-251 cells
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

ChIPSee 1 publications below

产品信息

21069-1-AP targets SIM2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用ChIP
经测试反应性 human, mouse
文献引用反应性human
免疫原 SIM2 fusion protein Ag15204 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 single-minded homolog 2 (Drosophila)
别名 bHLHe15, SIM, SIM2, Single minded homolog 2
计算分子量 667 aa, 73 kDa
观测分子量 60-64 kDa
GenBank蛋白编号BC110444
基因名称 SIM2
Gene ID (NCBI) 6493
RRIDAB_10732844
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14190
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

实验方案

Product Specific Protocols
WB protocol for SIM2 antibody 21069-1-APDownload protocol
IP protocol for SIM2 antibody 21069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanChIP

Biochem J

Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes.

Authors - Emily Lyn Button
View Article