验证数据展示
经过测试的应用
Positive WB detected in | mouse spleen tissue |
Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse spleen tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84365-5-RR targets SIGNR1/CD209b in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
经测试反应性 | mouse |
免疫原 | SIGNR1/CD209b fusion protein Eg1497 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | CD209b antigen |
别名 | 241726B1, CD209, CD209 antigen-like protein B, Cd209b, DC-SIGNR1 |
计算分子量 | 37 kDa |
观测分子量 | 45-50 kDa |
GenBank蛋白编号 | NM_026972.5 |
基因名称 | Cd209b |
Gene ID (NCBI) | 69165 |
RRID | AB_3671901 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q8CJ91-1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIGNR1 (CD209b) is a mouse homolog of the human DC-SIGN receptor (PMID: 11581173). SIGNR1 is a type II transmembrane protein with an extracellular domain consisting of a neck region made up of four repeats of 28 amino acids and a carbohydrate recognition domain (PMID: 15583012). It is expressed on marginal zone macrophages and peritoneal macrophages and is a key receptor for the recognition and efficient clearance of pathogens by the innate immune system.
实验方案
Product Specific Protocols | |
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WB protocol for SIGNR1/CD209b antibody 84365-5-RR | Download protocol |
IHC protocol for SIGNR1/CD209b antibody 84365-5-RR | Download protocol |
IF protocol for SIGNR1/CD209b antibody 84365-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |