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验证数据展示

  • WB analysis using 85064-1-RR (same clone as 85064-1-PBS)

    WB analysis using 85064-1-RR (same clone as 85064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85064-1-RR (SIGMAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85064-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85064-1-RR (same clone as 85064-1-PBS)

    IHC staining of mouse liver using 85064-1-RR (same clone as 85064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85064-1-RR (SIGMAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85064-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85064-1-RR (same clone as 85064-1-PBS)

    IF Staining of HeLa using 85064-1-RR (same clone as 85064-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIGMAR1 antibody (85064-1-RR, Clone: 242621C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85064-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01809-1

    Cytometric bead array standard curve of MP01809-1

    Cytometric bead array standard curve of MP01809-1, SIGMAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85064-2-PBS. Detection antibody: 85064-1-PBS. Standard: Ag7351. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 85064-1-RR

    Affinity and Kinetic Characterization of 85064-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85064-1-RR against Human SIGMAR1 were performed. The affinity constant is 1.54 nM.

SIGMAR1 Recombinant monoclonal antibody, PBS Only (Detector)

SIGMAR1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85064-1-PBS

产品说明书

CloneNo. 242621C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

242621C6, AAG8, Aging-associated gene 8 protein, hSigmaR1, OPRS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85064-1-PBS targets SIGMAR1 as part of a matched antibody pair:

MP01809-1: 85064-2-PBS capture and 85064-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7351

Product name: Recombinant human SIGMAR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-223 aa of BC004899

Sequence: SEYVLLFGTALGSRGHSGRYWAEISDTIISGTFHQWREGTTKSEVFYPGETVVHGPGEATAVEWGPNTWMVEYGRGVIPSTLAFALADTVFSTQDFLTLFYTLRSYARGLRLELTTYLFGQDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sigma non-opioid intracellular receptor 1
别名 242621C6, AAG8, Aging-associated gene 8 protein, hSigmaR1, OPRS1
计算分子量 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC004899
基因名称 SIGMAR1
Gene ID (NCBI) 10280
RRIDAB_3743982
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99720
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 25 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.

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