SIGMAR1 Recombinant antibody

SIGMAR1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85064-1-RR

产品说明书

CloneNo. 242621C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

242621C6, AAG8, Aging-associated gene 8 protein, hSigmaR1, OPRS1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293T cells, COLO 320 cells, C2C12 cells, C6 cells, mouse colon tissue, ratcolon tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85064-1-RR targets SIGMAR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SIGMAR1 fusion protein Ag7351 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sigma non-opioid intracellular receptor 1
别名 242621C6, AAG8, Aging-associated gene 8 protein, hSigmaR1, OPRS1
计算分子量 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC004899
基因名称 SIGMAR1
Gene ID (NCBI) 10280
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99720
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 25 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.

实验方案

Product Specific Protocols
WB protocol for SIGMAR1 antibody 85064-1-RRDownload protocol
IHC protocol for SIGMAR1 antibody 85064-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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