验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, THP-1 cells, U-937 cells |
| Positive IF/ICC detected in | U-937 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86628-3-RR targets SIGLEC14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg4178 Product name: Recombinant Human SIGLEC14 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 17-358 aa of NM_001098612.1 Sequence: EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQVKRQGAQVTTERTVQLNVSYAPQNLAISIFFRNGTGTALRILSNGMSVPIQEGQSLFLACTVDSNPPASLSWFREGKALNPSQTSMSGTLELPNIGAREGGEFTCRVQHPLGSQHLSFILSVQRSSSSCICVTEKQQGSWPLVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sialic acid binding Ig-like lectin 14 |
| 别名 | Siglec-14, Sialic acid-binding Ig-like lectin 14, Siglec 14, UNQ294/PRO333 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | NM_001098612.1 |
| 基因名称 | SIGLEC14 |
| Gene ID (NCBI) | 100049587 |
| RRID | AB_3745019 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q08ET2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Siglecs are involved in regulating cell proliferation, differentiation, apoptosis, and implementing cell-cell interactions (PMID: 19076345). They play important roles in regulating immune responses, in hostpathogen interactions, and in tumorigenesis (PMID: 22524423; 25234143). Sialic acid-binding Ig-like lectin 14 (SIGLEC14) is expressed by granulocytes and monocytes, which has an activating role in regulating macrophage immune responses (PMID: 24799499; 37024598).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SIGLEC14 antibody 86628-3-RR | Download protocol |
| WB protocol for SIGLEC14 antibody 86628-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
