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验证数据展示

  • WB analysis using 86628-3-RR (same clone as 86628-3-PBS)

    WB analysis using 86628-3-RR (same clone as 86628-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86628-3-RR (Siglec-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86628-3-PBS in a different storage buffer formulation.

  • IF Staining of U-937 using 86628-3-RR (same clone as 86628-3-PBS)

    IF Staining of U-937 using 86628-3-RR (same clone as 86628-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using SIGLEC14 antibody (86628-3-RR, Clone: 250756E4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86628-3-PBS in a different storage buffer formulation.

SIGLEC14 Recombinant monoclonal antibody, PBS Only

SIGLEC14 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86628-3-PBS

产品说明书

CloneNo. 250756E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Siglec-14, Sialic acid-binding Ig-like lectin 14, Siglec 14, UNQ294/PRO333

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86628-3-PBS targets SIGLEC14 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg4178

Product name: Recombinant Human SIGLEC14 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 17-358 aa of NM_001098612.1

Sequence: EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQVKRQGAQVTTERTVQLNVSYAPQNLAISIFFRNGTGTALRILSNGMSVPIQEGQSLFLACTVDSNPPASLSWFREGKALNPSQTSMSGTLELPNIGAREGGEFTCRVQHPLGSQHLSFILSVQRSSSSCICVTEKQQGSWPLVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sialic acid binding Ig-like lectin 14
别名 Siglec-14, Sialic acid-binding Ig-like lectin 14, Siglec 14, UNQ294/PRO333
计算分子量44 kDa
观测分子量44 kDa
GenBank蛋白编号NM_001098612.1
基因名称 SIGLEC14
Gene ID (NCBI) 100049587
RRIDAB_3745019
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ08ET2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Siglecs are involved in regulating cell proliferation, differentiation, apoptosis, and implementing cell-cell interactions (PMID: 19076345). They play important roles in regulating immune responses, in hostpathogen interactions, and in tumorigenesis (PMID: 22524423; 25234143). Sialic acid-binding Ig-like lectin 14 (SIGLEC14) is expressed by granulocytes and monocytes, which has an activating role in regulating macrophage immune responses (PMID: 24799499; 37024598).

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