验证数据展示
经过测试的应用
| Positive WB detected in | human plasma |
For other applications, we recommend the unconjugated version of this antibody, 83096-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83096 targets SHBG in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sex hormone-binding globulin |
| 别名 | ABP, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG |
| 计算分子量 | 402 aa, 44 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC101785 |
| 基因名称 | SHBG |
| Gene ID (NCBI) | 6462 |
| RRID | AB_3673765 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04278 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 SHBG antibody CL750-83096 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |