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CoraLite® Plus 488-conjugated SH3GLB2 Monoclonal antibody

SH3GLB2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B1A10

Cat No : CL488-68325

Print datasheet

Synonyms

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

验证数据展示

  • IF Staining of HeLa using CL488-68325

    IF Staining of HeLa using CL488-68325

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SH3GLB2 antibody (CL488-68325, Clone: 4B1A10 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SH3GLB2 Ready-To-Use IHC Kit. SH3GLB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68325-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68325 targets SH3GLB2 in IF applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen SH3GLB2 fusion protein Ag8932 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SH3-domain GRB2-like endophilin B2
Synonyms Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2
Calculated Molecular Weight 395 aa, 44 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC014635
Gene Symbol SH3GLB2
Gene ID (NCBI) 56904
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NR46
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SH3GLB2 antibody CL488-68325Download protocol
Standard Protocols
Click here to view our Standard Protocols