验证数据展示
经过测试的应用
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
FC | See 4 publications below |
CoIP | See 1 publications below |
产品信息
11871-1-AP targets SH2D1B in IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, IF/ICC, ELISA Application Description |
文献引用应用 | CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | SH2D1B fusion protein Ag2454 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | SH2 domain containing 1B |
别名 | EAT 2, EAT2, EAT-2, EWS/FLI1-activated transcript 2, SH2 domain containing 1B |
计算分子量 | 132 aa, 15 kDa |
观测分子量 | 15 kDa |
GenBank蛋白编号 | BC022407 |
基因名称 | SH2D1B |
Gene ID (NCBI) | 117157 |
RRID | AB_10603473 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O14796 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH2D1B, also named as EAT2, an adaptor expressed in innate immune cells, including natural killer (NK) cells. It plays a role in controlling signal transduction through at least four receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. EAT-2 overexpression would enhance the innate immune responses and also result in more potent Carcinoembryonic antigen-specific adaptive immune responses.
实验方案
Product Specific Protocols | |
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IHC protocol for SH2D1B antibody 11871-1-AP | Download protocol |
IF protocol for SH2D1B antibody 11871-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Immunity Cytomegalovirus infection drives adaptive epigenetic diversification of NK cells with altered signaling and effector function. | ||
Immunity Epigenetic modification and antibody-dependent expansion of memory-like NK cells in human cytomegalovirus-infected individuals. | ||
Mol Ther Anti-NKG2C/IL-15/anti-CD33 Killer Engager Directs Primary and iPSC-derived NKG2C+ NK cells to Specifically Target Myeloid Leukemia. | ||
J Immunol Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells. | ||
J Immunol T Cells Regulate Peripheral Naive Mature B Cell Survival by Cell-Cell Contact Mediated through SLAMF6 and SAP. |