SH2D1B Polyclonal antibody

SH2D1B Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 11871-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA and More (1)

EAT 2, EAT2, EAT-2, EWS/FLI1-activated transcript 2, SH2 domain containing 1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11871-1-AP targets SH2D1B in IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用CoIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 SH2D1B fusion protein Ag2454 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH2 domain containing 1B
别名 EAT 2, EAT2, EAT-2, EWS/FLI1-activated transcript 2, SH2 domain containing 1B
计算分子量 132 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC022407
基因名称 SH2D1B
Gene ID (NCBI) 117157
RRIDAB_10603473
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14796
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH2D1B, also named as EAT2, an adaptor expressed in innate immune cells, including natural killer (NK) cells. It plays a role in controlling signal transduction through at least four receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. EAT-2 overexpression would enhance the innate immune responses and also result in more potent Carcinoembryonic antigen-specific adaptive immune responses.

实验方案

Product Specific Protocols
IHC protocol for SH2D1B antibody 11871-1-APDownload protocol
IF protocol for SH2D1B antibody 11871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

Immunity

Cytomegalovirus infection drives adaptive epigenetic diversification of NK cells with altered signaling and effector function.

Authors - Heinrich Schlums
humanFC

Immunity

Epigenetic modification and antibody-dependent expansion of memory-like NK cells in human cytomegalovirus-infected individuals.

Authors - Jaewon Lee

J Exp Med

ARID5B regulates metabolic programming in human adaptive NK cells.

Authors - Frank Cichocki

Mol Ther

Anti-NKG2C/IL-15/anti-CD33 Killer Engager Directs Primary and iPSC-derived NKG2C+ NK cells to Specifically Target Myeloid Leukemia.

Authors - Emily Chiu
human

J Immunol

Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells.

Authors - Hagberg Niklas N
humanFC,CoIP

J Immunol

T Cells Regulate Peripheral Naive Mature B Cell Survival by Cell-Cell Contact Mediated through SLAMF6 and SAP.

Authors - Lihi Radomir
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