SH2D1B Polyclonal antibody
SH2D1B Polyclonal Antibody for FC, IF, IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF, IHC,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
FC | See 4 publications below |
CoIP | See 1 publications below |
产品信息
11871-1-AP targets SH2D1B in IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC,ELISA |
Cited Applications | FC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | SH2D1B fusion protein Ag2454 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SH2 domain containing 1B |
Synonyms | EAT 2, EAT2, SH2 domain containing 1B, SH2D1B |
Calculated Molecular Weight | 132 aa, 15 kDa |
Observed Molecular Weight | 15 kDa |
GenBank Accession Number | BC022407 |
Gene Symbol | SH2D1B |
Gene ID (NCBI) | 117157 |
RRID | AB_10603473 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14796 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for SH2D1B antibody 11871-1-AP | Download protocol |
IF protocol for SH2D1B antibody 11871-1-AP | Download protocol |
FC protocol for SH2D1B antibody 11871-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Immunity Cytomegalovirus infection drives adaptive epigenetic diversification of NK cells with altered signaling and effector function. | ||
Immunity Epigenetic modification and antibody-dependent expansion of memory-like NK cells in human cytomegalovirus-infected individuals. | ||
Mol Ther Anti-NKG2C/IL-15/anti-CD33 Killer Engager Directs Primary and iPSC-derived NKG2C+ NK cells to Specifically Target Myeloid Leukemia. | ||
J Immunol Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells. | ||
J Immunol T Cells Regulate Peripheral Naive Mature B Cell Survival by Cell-Cell Contact Mediated through SLAMF6 and SAP. |