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验证数据展示

  • IF Staining of HeLa using CL594-60305

    IF Staining of HeLa using CL594-60305

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 SGTA antibody (CL594-60305, Clone: 3B10B11 ) at dilution of 1:200.

CoraLite®594-conjugated SGTA Monoclonal antibody

SGTA Monoclonal Antibody for IF/ICC
Cat No. CL594-60305

产品说明书

CloneNo. 3B10B11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC

别名

hSGT, aSGT, Alpha-SGT, alphaSGT, Alpha SGT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SGTA Ready-To-Use IHC Kit. SGTA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60305-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60305 targets SGTA in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1475

Product name: Recombinant human SGTA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC008885

Sequence: MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
别名 hSGT, aSGT, Alpha-SGT, alphaSGT, Alpha SGT
计算分子量 34 kDa
观测分子量 34-37 kDa
GenBank蛋白编号BC008885
基因名称 SGTA
Gene ID (NCBI) 6449
RRIDAB_2883459
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43765
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.

实验方案

Product Specific Protocols
IF protocol for CL594 SGTA antibody CL594-60305Download protocol
Standard Protocols
Click here to view our Standard Protocols