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CoraLite® Plus 488-conjugated SGTA Monoclonal antibody

SGTA Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B10B11

Cat No : CL488-60305

Print datasheet

Synonyms

Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein

验证数据展示

  • IF Staining of HeLa using CL488-60305

    IF Staining of HeLa using CL488-60305

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 SGTA antibody (CL488-60305, Clone: 3B10B11 ) at dilution of 1:400.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SGTA Ready-To-Use IHC Kit. SGTA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60305-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60305 targets SGTA in IF applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse
Immunogen SGTA fusion protein Ag1475 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
Synonyms Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34-37 kDa
GenBank Accession NumberBC008885
Gene Symbol SGTA
Gene ID (NCBI) 6449
RRIDAB_2883139
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43765
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SGTA antibody CL488-60305Download protocol
Standard Protocols
Click here to view our Standard Protocols