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验证数据展示

  • WB analysis of A549 using 13550-1-AP (same clone as 13550-1-PBS)

    WB analysis of A549 using 13550-1-AP (same clone as 13550-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • WB analysis using 13550-1-AP (same clone as 13550-1-PBS)

    WB analysis using 13550-1-AP (same clone as 13550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13550-1-AP (same clone as 13550-1-PBS)

    IF Staining of HepG2 using 13550-1-AP (same clone as 13550-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 13550-1-AP (SFI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SFI1 Polyclonal antibody, PBS Only

SFI1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13550-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

hSFI1, KIAA0542, Protein SFI1 homolog

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13550-1-PBS targets SFI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4268

Product name: Recombinant human SFI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 917-1211 aa of BC033613

Sequence: MAQAHPSLLADLGLAFPDFQSPYKIGDPTEISDPPAAPGQPRGTDPMFLSSNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sfi1 homolog, spindle assembly associated (yeast)
别名 hSFI1, KIAA0542, Protein SFI1 homolog
计算分子量 1211 aa, 134 kDa
观测分子量 148 kDa
GenBank蛋白编号BC033613
基因名称 SFI1
Gene ID (NCBI) 9814
RRIDAB_10596938
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA8K8P3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

human SFI1 is a centriolar protein that associates with a pool of Centrin at the distal end of the centriole. SFI1 forms an interdependent complex with centrin at the centriolar distal end. Human SFI1 is an evolutionarily conserved protein of 1,242 amino acids that contains five residues within full Cdk consensus motifs (S/T*-P-x-K/R), four at the C terminus known to be phosphorylated in vivo (T816, S855, S882, S892) and one immediately preceding the C terminus (PMID: 25340401, 31197030).

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