验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, A549 cells, HepG2 cells, mouse lung tissue, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13550-1-AP targets SFI1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4268 Product name: Recombinant human SFI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 917-1211 aa of BC033613 Sequence: MAQAHPSLLADLGLAFPDFQSPYKIGDPTEISDPPAAPGQPRGTDPMFLSSNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Sfi1 homolog, spindle assembly associated (yeast) |
| 别名 | hSFI1, KIAA0542, Protein SFI1 homolog |
| 计算分子量 | 1211 aa, 134 kDa |
| 观测分子量 | 148 kDa |
| GenBank蛋白编号 | BC033613 |
| 基因名称 | SFI1 |
| Gene ID (NCBI) | 9814 |
| RRID | AB_10596938 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A8K8P3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
human SFI1 is a centriolar protein that associates with a pool of Centrin at the distal end of the centriole. SFI1 forms an interdependent complex with centrin at the centriolar distal end. Human SFI1 is an evolutionarily conserved protein of 1,242 amino acids that contains five residues within full Cdk consensus motifs (S/T*-P-x-K/R), four at the C terminus known to be phosphorylated in vivo (T816, S855, S882, S892) and one immediately preceding the C terminus (PMID: 25340401, 31197030).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SFI1 antibody 13550-1-AP | Download protocol |
| WB protocol for SFI1 antibody 13550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Time-series reconstruction of the molecular architecture of human centriole assembly | ||
Sci Adv Centrin-POC5 inner scaffold provides distal centriole integrity for sperm flagellar assembly. | ||
EBioMedicine PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response | ||
EMBO J Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis
| ||
Sci Signal Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins. | ||
J Cell Biol SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL. |
