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SFI1 Polyclonal antibody

SFI1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13550-1-AP

Print datasheet

Synonyms

hSFI1, hSfi1p, KIAA0542, PISD, Protein SFI1 homolog, RP5 858B16.1, SFI1

验证数据展示

  • WB analysis of A549 using 13550-1-AP

    WB analysis of A549 using 13550-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13550-1-AP

    IF Staining of HepG2 using 13550-1-AP

    Immunofluorescent analysis of HepG2 cells using 13550-1-AP (SFI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, mouse lung tissue
Positive IF detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13550-1-AP targets SFI1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen SFI1 fusion protein Ag4268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sfi1 homolog, spindle assembly associated (yeast)
Synonyms hSFI1, hSfi1p, KIAA0542, PISD, Protein SFI1 homolog, RP5 858B16.1, SFI1
Calculated Molecular Weight 1211 aa, 134 kDa
Observed Molecular Weight 148 kDa
GenBank Accession NumberBC033613
Gene Symbol SFI1
Gene ID (NCBI) 9814
RRIDAB_10596938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA8K8P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SFI1 antibody 13550-1-APDownload protocol
IF protocol for SFI1 antibody 13550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL.

Authors - Andrew Kodani
View Article
humanWB

Sci Signal

Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins.

Authors - Isha Nasa
View Article
mouseWB

Sci Rep

Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation.

Authors - Akiko Matsuura
View Article
humanWB

J Cell Sci

Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair.

Authors - Ebtissal M Khouj
View Article
humanIF

EMBO J

Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis

Authors - Marine H Laporte
  • KD Validated
View Article