验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, mouse lung tissue |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13550-1-AP targets SFI1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4268 Product name: Recombinant human SFI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 917-1211 aa of BC033613 Sequence: MAQAHPSLLADLGLAFPDFQSPYKIGDPTEISDPPAAPGQPRGTDPMFLSSNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Sfi1 homolog, spindle assembly associated (yeast) |
| 别名 | Protein SFI1 homolog, KIAA0542, hSFI1 |
| 计算分子量 | 1211 aa, 134 kDa |
| 观测分子量 | 148 kDa |
| GenBank蛋白编号 | BC033613 |
| 基因名称 | SFI1 |
| Gene ID (NCBI) | 9814 |
| RRID | AB_10596938 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A8K8P3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SFI1 antibody 13550-1-AP | Download protocol |
| WB protocol for SFI1 antibody 13550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EBioMedicine PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response | ||
J Cell Biol SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL. | ||
J Cell Biol CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity | ||
Sci Signal Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins. | ||
Sci Rep Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation. | ||
J Cell Sci Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair. |
