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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F3B2

Cat No : CL594-60075

Print datasheet

Synonyms

DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, Pnutl3, SEPT2, Septin 2, septin2

验证数据展示

  • FC experiment of K-562 using CL594-60075

    FC experiment of K-562 using CL594-60075

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Septin 2 (CL594-60075, Clone:2F3B2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60075-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60075 targets Septin 2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen Septin 2 fusion protein Ag1946 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name septin 2
Synonyms DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, Pnutl3, SEPT2, Septin 2, septin2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC014455
Gene Symbol SEPT2
Gene ID (NCBI) 4735
RRIDAB_2883416
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15019
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

实验方案

Product Specific Protocols
FC protocol for CL594 Septin 2 antibody CL594-60075Download protocol
Standard Protocols
Click here to view our Standard Protocols