• Featured Product
  • KD/KO Validated

Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat


FC, IF/ICC, IHC, WB, ELISA and More (1)





Cat No : 60075-1-Ig

Print datasheet


DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, SEPT2, SEPT2/septin 2, Septin 2, septin2


Positive WB detected inLNCaP cells, Jurkat cells, fetal human brain tissue, human testis tissue, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, HSCT6 cells, ROS1728 cells, Neuro-2A cells
Positive IHC detected inhuman renal cell carcinoma tissue, human brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE cells, K-562 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.


Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


60075-1-Ig targets Septin 2 in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Septin 2 fusion protein Ag1946 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name septin 2
Synonyms DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, SEPT2, SEPT2/septin 2, Septin 2, septin2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC014455
Gene Symbol SEPT2
Gene ID (NCBI) 4735
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)


Product Specific Protocols
WB protocol for Septin 2 antibody 60075-1-IgDownload protocol
IHC protocol for Septin 2 antibody 60075-1-IgDownload protocol
IF protocol for Septin 2 antibody 60075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR

Cell Res

Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.

Authors - Lei Wang

J Cell Biol

Septins suppress the release of vaccinia virus from infected cells.

Authors - Julia Pfanzelter

Cell Rep

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Authors - Fernando Calvo


Contribution of septins to human platelet structure and function.

Authors - Oleg V Kim

Cytoskeleton (Hoboken)

A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.

Authors - Damián Lobato-Márquez
  • KD Validated