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验证数据展示

  • WB analysis using 83594-4-RR

    WB analysis using 83594-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 83594-4-RR

    IHC staining of human ovarian cancer using 83594-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83594-4-RR

    Affinity and Kinetic Characterization of 83594-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

SENP1 Recombinant antibody

SENP1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83594-4-RR

产品说明书

CloneNo. 240478D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HL-60 cells, Jurkat cells, rat testis tiuuse
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83594-4-RR targets SENP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SENP1 fusion protein Ag18028 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SUMO1/sentrin specific peptidase 1
别名 SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3
计算分子量 644 aa, 73 kDa
观测分子量73 kDa
GenBank蛋白编号BC045639
基因名称 SENP1
Gene ID (NCBI) 29843
RRIDAB_3671210
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P0U3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

实验方案

Product Specific Protocols
WB protocol for SENP1 antibody 83594-4-RRDownload protocol
IHC protocol for SENP1 antibody 83594-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols