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验证数据展示

  • WB analysis using 83594-4-RR

    WB analysis using 83594-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 83594-4-RR

    IHC staining of human ovarian cancer using 83594-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SENP1 antibody included
  • Affinity and Kinetic Characterization of 83594-4-RR

    Affinity and Kinetic Characterization of 83594-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

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SENP1 Recombinant monoclonal antibody

SENP1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83594-4-RR

产品说明书

CloneNo. 240478D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HL-60 cells, Jurkat cells, rat testis tiuuse
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® SENP1 Ready-To-Use IHC Kit. SENP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83594-4-RR targets SENP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18028

Product name: Recombinant human SENP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-331 aa of BC045639

Sequence: MDDIADRMRMDAGEVTLVNHNSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAAYNPSYYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSRSLYLETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRRSLLSPKKTQRRHVSTAEETVQEEEREVYRQLLQMVTGKQFTIAKPTTHFPLHLSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVILLKVKDSQTPTP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SUMO1/sentrin specific peptidase 1
别名 SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3
计算分子量 644 aa, 73 kDa
观测分子量73 kDa
GenBank蛋白编号BC045639
基因名称 SENP1
Gene ID (NCBI) 29843
RRIDAB_3671210
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P0U3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

实验方案

Product Specific Protocols
WB protocol for SENP1 antibody 83594-4-RRDownload protocol
IHC protocol for SENP1 antibody 83594-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols