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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 84080-3-RR (same clone as 84080-3-PBS)

    WB analysis using 84080-3-RR (same clone as 84080-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84080-3-RR (SEMA3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84080-3-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 84080-3-RR (same clone as 84080-3-PBS)

    IF Staining of U-251 using 84080-3-RR (same clone as 84080-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SEMA3B antibody (84080-3-RR, Clone: 241171A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84080-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84080-3-RR

    Affinity and Kinetic Characterization of 84080-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84080-3-RR against Human SEMA3B were performed. The affinity constant is 8.53 nM.

SEMA3B Recombinant antibody, PBS Only

SEMA3B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84080-3-PBS

产品说明书

CloneNo. 241171A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

241171A7, LUCA 1, SemA, Sema A(V), Sema V

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84080-3-PBS targets SEMA3B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SEMA3B fusion protein Ag25163 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
别名 241171A7, LUCA 1, SemA, Sema A(V), Sema V
观测分子量83 kDa
GenBank蛋白编号BC009113
基因名称 SEMA3B
Gene ID (NCBI) 7869
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13214
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEMA3B is a class 3 semaphorin/collapsin family member containing 749 highly conserved, NH2 terminal, semaphorin amino acids. SEMA3B is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. SEMA3B has been found in lung cancer, ovarian cancer, hepatocellular carcinoma, and cholangiocarcinoma. SEMA3B is expressed abundantly but differentially in various neural and nonneural tissues. The calculated molecular weight of SEMA3B is 83 kDa (PMID: 30914894, PMID: 32410560).