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验证数据展示

  • WB analysis using 84080-3-RR

    WB analysis using 84080-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84080-3-RR (SEMA3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 84080-3-RR

    IF Staining of U-251 using 84080-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SEMA3B antibody (84080-3-RR, Clone: 241171A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84080-3-RR

    Affinity and Kinetic Characterization of 84080-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84080-3-RR against Human SEMA3B were performed. The affinity constant is 8.53 nM.

SEMA3B Recombinant antibody

SEMA3B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84080-3-RR

产品说明书

CloneNo. 241171A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

241171A7, LUCA 1, SemA, Sema A(V), Sema V

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, U-251 cells, SK-N-SH cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84080-3-RR targets SEMA3B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SEMA3B fusion protein Ag25163 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
别名 241171A7, LUCA 1, SemA, Sema A(V), Sema V
观测分子量83 kDa
GenBank蛋白编号BC009113
基因名称 SEMA3B
Gene ID (NCBI) 7869
RRIDAB_3671648
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13214
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEMA3B is a class 3 semaphorin/collapsin family member containing 749 highly conserved, NH2 terminal, semaphorin amino acids. SEMA3B is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. SEMA3B has been found in lung cancer, ovarian cancer, hepatocellular carcinoma, and cholangiocarcinoma. SEMA3B is expressed abundantly but differentially in various neural and nonneural tissues. The calculated molecular weight of SEMA3B is 83 kDa (PMID: 30914894, PMID: 32410560).

实验方案

Product Specific Protocols
WB protocol for SEMA3B antibody 84080-3-RRDownload protocol
IF protocol for SEMA3B antibody 84080-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols