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验证数据展示

  • WB analysis using 31489-1-AP (same clone as 31489-1-PBS)

    WB analysis using 31489-1-AP (same clone as 31489-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31489-1-AP (SEL1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31489-1-PBS in a different storage buffer formulation.

  • IP experiment of Daudi using 31489-1-AP (same clone as 31489-1-PBS)

    IP experiment of Daudi using 31489-1-AP (same clone as 31489-1-PBS)

    IP result of anti-SEL1L3 (IP:31489-1-AP, 4ug; Detection:31489-1-AP 1:1000) with Daudi cells lysate 1600 ug. This data was developed using the same antibody clone with 31489-1-PBS in a different storage buffer formulation.

  • IP experiment of U-251 using 31489-1-AP (same clone as 31489-1-PBS)

    IP experiment of U-251 using 31489-1-AP (same clone as 31489-1-PBS)

    IP result of anti-SEL1L3 (IP:31489-1-AP, 4ug; Detection:31489-1-AP 1:1000) with U-251 cells lysate 1320 ug. This data was developed using the same antibody clone with 31489-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 31489-1-AP (same clone as 31489-1-PBS)

    IHC staining of human colon cancer using 31489-1-AP (same clone as 31489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31489-1-AP (SEL1L3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31489-1-PBS in a different storage buffer formulation.

SEL1L3 Polyclonal antibody, PBS Only

SEL1L3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 31489-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

KIAA0746, Protein sel 1 homolog 3, Sel 1L3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31489-1-PBS targets SEL1L3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34856

Product name: Recombinant human SEL1L3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-236 aa of BC060867

Sequence: KYSGRDWNVKWEENLLHAVAKNYTLLQTIPPFERPFKDHQVCLEWNMGYIWNLRANRIPQCPLENDVVALLGFPYASSGENTGIVKKFPRFRNRELEATRRQRMDYPVFTVSLWLYLLHYCKANLCGILYFVDSNEMYGTPSVFLTEEGYLHIQMHLVKGEDLAVKTKFIIPLKEWFRLDISFNGGQIVVTTSIGQDLKSYHNQTISFRE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0746 protein
别名 KIAA0746, Protein sel 1 homolog 3, Sel 1L3
观测分子量 129 kDa
GenBank蛋白编号BC060867
基因名称 KIAA0746
Gene ID (NCBI) 23231
RRIDAB_3742437
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ68CR1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEL1L3 is also named suppressor of lin-12-like protein 3. SEL1L3, a protein with multiple glycosylation sites, was identified as the BCR target in 3/20 PVRL cases. SEL1L3 induces proliferation and BCR pathway activation in aggressive lymphoma cell lines. Moreover, SEL1L3 conjugated to a toxin killed exclusively lymphoma cells with respective BCR-reactivity (PMID: 38671086).

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