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验证数据展示

  • WB analysis using 31489-1-AP

    WB analysis using 31489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31489-1-AP (SEL1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Daudi using 31489-1-AP

    IP experiment of Daudi using 31489-1-AP

    IP result of anti-SEL1L3 (IP:31489-1-AP, 4ug; Detection:31489-1-AP 1:1000) with Daudi cells lysate 1600 ug.

  • IP experiment of U-251 using 31489-1-AP

    IP experiment of U-251 using 31489-1-AP

    IP result of anti-SEL1L3 (IP:31489-1-AP, 4ug; Detection:31489-1-AP 1:1000) with U-251 cells lysate 1320 ug.

  • IHC staining of human colon cancer using 31489-1-AP

    IHC staining of human colon cancer using 31489-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31489-1-AP (SEL1L3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SEL1L3 Polyclonal antibody

SEL1L3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 31489-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

KIAA0746, Protein sel 1 homolog 3, Sel 1L3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, Ramos cells, U-251 cells
Positive IP detected inDaudi cells, U-251 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31489-1-AP targets SEL1L3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34856

Product name: Recombinant human SEL1L3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-236 aa of BC060867

Sequence: KYSGRDWNVKWEENLLHAVAKNYTLLQTIPPFERPFKDHQVCLEWNMGYIWNLRANRIPQCPLENDVVALLGFPYASSGENTGIVKKFPRFRNRELEATRRQRMDYPVFTVSLWLYLLHYCKANLCGILYFVDSNEMYGTPSVFLTEEGYLHIQMHLVKGEDLAVKTKFIIPLKEWFRLDISFNGGQIVVTTSIGQDLKSYHNQTISFRE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0746 protein
别名 KIAA0746, Protein sel 1 homolog 3, Sel 1L3
观测分子量 129 kDa
GenBank蛋白编号BC060867
基因名称 KIAA0746
Gene ID (NCBI) 23231
RRIDAB_3742437
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ68CR1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEL1L3 is also named suppressor of lin-12-like protein 3. SEL1L3, a protein with multiple glycosylation sites, was identified as the BCR target in 3/20 PVRL cases. SEL1L3 induces proliferation and BCR pathway activation in aggressive lymphoma cell lines. Moreover, SEL1L3 conjugated to a toxin killed exclusively lymphoma cells with respective BCR-reactivity (PMID: 38671086).

实验方案

Product Specific Protocols
IHC protocol for SEL1L3 antibody 31489-1-APDownload protocol
IP protocol for SEL1L3 antibody 31489-1-APDownload protocol
WB protocol for SEL1L3 antibody 31489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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