验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HepG2 cells, MCF-7 cells, BxPC-3 cells, U-87 MG cells, mouse kidney tissue, rat liver tissue |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84317-7-RR targets SEL1L in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag32178 Product name: Recombinant human SEL1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-225 aa of NM_005065 Sequence: SSDEEGSQDESLDSKTTLTSDESVKDHTTAGRVVAGQIFLDSEESELESSIQEEEDSLKSQEGESVTEDISFLESPNPENKDYEEPKKVRKPALTAIEGTAHGEPCHFPFLFLDKEYDECTSDGREDGRLWCATTYDYKADEKWGFCETEEEAAKRRQMQEAEMMYQTGMKILNGSNKKSQKREAYRYLQKAASMNHTKALERV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sel-1 suppressor of lin-12-like (C. elegans) |
| 别名 | 241594G2, Protein sel 1 homolog 1, Protein sel-1 homolog 1, Sel 1L, Sel-1L |
| 计算分子量 | 89 kDa |
| 观测分子量 | 89 kDa |
| GenBank蛋白编号 | NM_005065 |
| 基因名称 | SEL1L |
| Gene ID (NCBI) | 6400 |
| RRID | AB_3671858 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9UBV2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen. .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEL1L antibody 84317-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
