SECTM1 Monoclonal antibody
SECTM1 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human
Applications
FC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C11B4
验证数据展示
经过测试的应用
| Positive WB detected in | HaCaT cells, JAR cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | K-562 cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
60281-1-Ig targets SECTM1 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | SECTM1 fusion protein Ag14645 种属同源性预测 |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | secreted and transmembrane 1 |
| Synonyms | K12, Protein K 12, secreted and transmembrane 1, SECTM1 |
| Calculated Molecular Weight | 248 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC017716 |
| Gene Symbol | SECTM1 |
| Gene ID (NCBI) | 6398 |
| RRID | AB_2881399 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| UNIPROT ID | Q8WVN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SECTM1 antibody 60281-1-Ig | Download protocol |
| IHC protocol for SECTM1 antibody 60281-1-Ig | Download protocol |
| FC protocol for SECTM1 antibody 60281-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience SECTM1 is upregulated in immuno-hot tumors and predicts immunotherapeutic efficacy in multiple cancers |
