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验证数据展示

  • WB analysis using 60281-1-Ig (same clone as 60281-1-PBS)

    WB analysis using 60281-1-Ig (same clone as 60281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60281-1-Ig (SECTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

SECTM1 Monoclonal antibody, PBS Only

SECTM1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60281-1-PBS

产品说明书

CloneNo. 1C11B4

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

K12, Protein K 12, secreted and transmembrane 1, SECTM1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

60281-1-PBS targets SECTM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14645

Product name: Recombinant human SECTM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 29-248 aa of BC017716

Sequence: QNEGWDSPICTEGVVSVSWGENTVMSCNISNAFSHVNIKLRAHGQESAIFNEVAPGYFSRDGWQLQVQGGVAQLVIKGARDSHAGLYMWHLVGHQRNNRQVTLEVSGAEPQSAPDTGFWPVPAVVTAVFILLVALVMFAWYRCRCSQQRREKKFFLLEPQMKFAALRAGAQQGLSRASAELWTPDSEPTPRPLALVFKPSPLGALELLSPQPLFPYAADP

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 secreted and transmembrane 1
别名 K12, Protein K 12, secreted and transmembrane 1, SECTM1
计算分子量 248 aa, 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC017716
基因名称 SECTM1
Gene ID (NCBI) 6398
RRIDAB_2881399
偶联类型 Unconjugated
形式Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDQ8WVN6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.