CoraLite® Plus 488-conjugated SEC63 Monoclonal antibody
SEC63 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C10E1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 67352-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67352 targets SEC63 in IF/ICC applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human |
| Immunogen | SEC63 fusion protein Ag5783 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SEC63 homolog (S. cerevisiae) |
| Synonyms | ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC047221 |
| Gene Symbol | SEC63 |
| Gene ID (NCBI) | 11231 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UGP8 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SEC63 antibody CL488-67352 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |