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验证数据展示

  • WB analysis using 67352-1-Ig

    WB analysis using 67352-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67352-1-Ig (SEC63 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67352-1-Ig

    IF Staining of HEK-293 using 67352-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SEC63 antibody (67352-1-Ig, Clone: 1C10E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SEC63 Monoclonal antibody

SEC63 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67352-1-Ig

产品说明书

CloneNo. 1C10E1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67352-1-Ig targets SEC63 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 SEC63 fusion protein Ag5783 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SEC63 homolog (S. cerevisiae)
别名 ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L
计算分子量 88 kDa
观测分子量 90 kDa
GenBank蛋白编号BC047221
基因名称 SEC63
Gene ID (NCBI) 11231
RRIDAB_2882608
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UGP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC63 is a integral endoplasmic reticulum (ER) membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

实验方案

Product Specific Protocols
WB protocol for SEC63 antibody 67352-1-IgDownload protocol
IF protocol for SEC63 antibody 67352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols