验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SEC31A Ready-To-Use IHC Kit. SEC31A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84942-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-84942-4 targets SEC31A in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | SEC31A fusion protein Ag12314 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SEC31 homolog A (S. cerevisiae) |
| 别名 | 242531E2, ABP125, ABP130, KIAA0905, SEC31 like protein 1 |
| 计算分子量 | 1220 aa, 133 kDa |
| 观测分子量 | 118-140 kDa |
| GenBank蛋白编号 | BC084583 |
| 基因名称 | SEC31A |
| Gene ID (NCBI) | 22872 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O94979 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). The sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologs of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 SEC31A antibody CL647-84942-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |