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验证数据展示

  • WB analysis using 84942-4-RR (same clone as 84942-4-PBS)

    WB analysis using 84942-4-RR (same clone as 84942-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84942-4-RR (SEC31A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84942-4-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 84942-4-RR (same clone as 84942-4-PBS)

    IP experiment of HeLa using 84942-4-RR (same clone as 84942-4-PBS)

    IP result of anti-SEC31A (IP:84942-4-RR, 4ug; Detection:84942-4-RR 1:4000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 84942-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84942-4-RR (same clone as 84942-4-PBS)

    IF Staining of HeLa using 84942-4-RR (same clone as 84942-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SEC31A antibody (84942-4-RR, Clone: 242531E2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84942-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84942-4-RR

    Affinity and Kinetic Characterization of 84942-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84942-4-RR against Human SEC31A were performed. The affinity constant is 12.4 nM.

SEC31A Recombinant antibody, PBS Only

SEC31A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 84942-4-PBS

产品说明书

CloneNo. 242531E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

242531E2, ABP125, ABP130, KIAA0905, SEC31 like protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SEC31A Ready-To-Use IHC Kit. SEC31A primary antibody included.

产品信息

84942-4-PBS targets SEC31A in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 SEC31A fusion protein Ag12314 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SEC31 homolog A (S. cerevisiae)
别名 242531E2, ABP125, ABP130, KIAA0905, SEC31 like protein 1
计算分子量 1220 aa, 133 kDa
观测分子量 118-140 kDa
GenBank蛋白编号BC084583
基因名称 SEC31A
Gene ID (NCBI) 22872
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO94979
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). The sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologs of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.