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验证数据展示

  • WB analysis using 84942-4-RR

    WB analysis using 84942-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84942-4-RR (SEC31A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 84942-4-RR

    IP experiment of HeLa using 84942-4-RR

    IP result of anti-SEC31A (IP:84942-4-RR, 4ug; Detection:84942-4-RR 1:4000) with HeLa cells lysate 1880 ug.

  • IF Staining of HeLa using 84942-4-RR

    IF Staining of HeLa using 84942-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SEC31A antibody (84942-4-RR, Clone: 242531E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84942-4-RR

    Affinity and Kinetic Characterization of 84942-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84942-4-RR against Human SEC31A were performed. The affinity constant is 12.4 nM.

SEC31A Recombinant monoclonal antibody

SEC31A Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 84942-4-RR

产品说明书

CloneNo. 242531E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

242531E2, ABP125, ABP130, KIAA0905, SEC31 like protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SEC31A Ready-To-Use IHC Kit. SEC31A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84942-4-RR targets SEC31A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12314

Product name: Recombinant human SEC31A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 758-1106 aa of BC084583

Sequence: AAQGSIAAALAFLPDNTNQPNIMQLRDRLCRAQGEPVAGHESPKIPYEKQQLPKGRPGPVAGHHQMPRVQTQQYYPHGENPPPPGFIMHGNVNPNAAGQLPTSPGHMHTQVPPYPQPQRPQNGWNDPPALNRVPKKKKMPENFMPPVPITSPIMNPLGDPQSQMLQQQPSAPVPLSSQSSFPQPHLPGGQPFHGVQQPLGQTGMPPSFSKPNIEGAPGAPIGNTFQHVQSLPTKKITKKPIPDEHLILKTTFEDLIQRCLSSATDPQTKRKLDDASKRLEFLYDKLREQTLSPTITSGLHNIARSIETRNYSEGLTMHTHIVSTSNFSETSAFMPVLKVVLTQANKLGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SEC31 homolog A (S. cerevisiae)
别名 242531E2, ABP125, ABP130, KIAA0905, SEC31 like protein 1
计算分子量 1220 aa, 133 kDa
观测分子量 118-140 kDa
GenBank蛋白编号BC084583
基因名称 SEC31A
Gene ID (NCBI) 22872
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO94979
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). The sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologs of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

实验方案

Product Specific Protocols
IF protocol for SEC31A antibody 84942-4-RRDownload protocol
IP protocol for SEC31A antibody 84942-4-RRDownload protocol
WB protocol for SEC31A antibody 84942-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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