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验证数据展示

  • IF Staining of HeLa using CL488-82908

    IF Staining of HeLa using CL488-82908

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SEC24A antibody (CL488-82908, Clone: 230105B3 ) at dilution of 1:200.

  • FC experiment of Hela using CL488-82908

    FC experiment of Hela using CL488-82908

    1x10^6 Hela cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sec24a Recombinant Antibody (CL488-82908, Clone:230105B3)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated SEC24A Recombinant monoclonal antibody

SEC24A Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82908

产品说明书

CloneNo. 230105B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

SEC24-related protein A, Protein transport protein Sec24A, 230105B3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHela cells
For other applications, we recommend the unconjugated version of this antibody, 82908-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82908 targets SEC24A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag8723

Product name: Recombinant human SEC24A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 264-613 aa of BC019341

Sequence: DEIEGGGLLATPQLTNKNPKMSRSVGYSYPSLPPGYQNITPPGATGVPPSSLNYPSGPQAFTQTPLGANHLTTSMSGLSLQPEGLRVVNLLQERNMLPSTPLKPPVPNLHEDIQKLNCNPELFRCTLTSIPQMQALLNKAKLPLGLLLHPFKDLVQLPVVTSSTIVRCRSCRTYINPFVSFLDQRRWKCNLCYRVNDVPEEFLYNPLTRVYGEPHRRPEVQNATIEFMAPSEYMLRPPQPPVYLFVFDVSHNAVETGYLNSVCQSLLDNLDLLPGNTRTKIGFITFDSTIHFYGLQESLSQPQMLIVSDIEDVFIPMPENLLVNLNESKESVIGVSSEETLITCLEIAMR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SEC24 family, member A (S. cerevisiae)
别名 SEC24-related protein A, Protein transport protein Sec24A, 230105B3
计算分子量 613 aa, 66 kDa
观测分子量120 kDa
GenBank蛋白编号BC019341
基因名称 SEC24A
Gene ID (NCBI) 10802
RRIDAB_3673109
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95486
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SEC24A antibody CL488-82908Download protocol
FC protocol for CL Plus 488 SEC24A antibody CL488-82908Download protocol
Standard Protocols
Click here to view our Standard Protocols