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SEC24A Recombinant antibody

SEC24A Recombinant Antibody for WB,IF/ICC,FC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,IF/ICC,FC,ELISA

Conjugate

Unconjugated

CloneNo.

230105B3

Cat No : 82908-1-RR

Print datasheet

Synonyms

SEC24 related protein A, SEC24A

验证数据展示

  • WB analysis using 82908-1-RR

    WB analysis using 82908-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82908-1-RR (SEC24A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82908-1-RR

    IF Staining of HeLa using 82908-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC24A antibody (82908-1-RR, Clone: 230105B3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 82908-1-RR

    FC experiment of HepG2 using 82908-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SEC24A (82908-1-RR, Clone:230105B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inU-87 MG cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:125-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82908-1-RR targets SEC24A in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB,IF/ICC,FC,ELISA
Tested Reactivity Human
Immunogen SEC24A fusion protein Ag8723 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SEC24 family, member A (S. cerevisiae)
Synonyms SEC24 related protein A, SEC24A
Calculated Molecular Weight 613 aa, 66 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC019341
Gene Symbol SEC24A
Gene ID (NCBI) 10802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95486
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.

实验方案

Product Specific Protocols
WB protocol for SEC24A antibody 82908-1-RRDownload protocol
IF protocol for SEC24A antibody 82908-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols