• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83319-1-RR (same clone as 83319-1-PBS)

    WB analysis using 83319-1-RR (same clone as 83319-1-PBS)

    Untreated and DENSPM treated HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 83319-1-RR (same clone as 83319-1-PBS)

    WB analysis of K-562 using 83319-1-RR (same clone as 83319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00344-4

    Sandwich ELISA standard curve of MP00344-4

    Sandwich ELISA standard curve of MP00344-4, Human SAT1 Recombinant Matched Antibody Pair - PBS only. 83319-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1154. 83319-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00344-4

    Sample test of MP00344-4

    The mean SAT1 concentration was determined to be 60.9 ng/mL in DENSPM-stimulated HeLa cell extracts based on a 3.0 mg/mL extract load.

  • Sample test of MP00344-4

    Sample test of MP00344-4

    The mean SAT1 concentration was determined to be 62.4 ng/mL in U-251 cell extract based on a 1.4 mg/mL extract load.

  • IHC staining of mouse brain using 83319-1-RR (same clone as 83319-1-PBS)

    IHC staining of mouse brain using 83319-1-RR (same clone as 83319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83319-1-RR (SAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83319-1-RR (same clone as 83319-1-PBS)

    IHC staining of mouse brain using 83319-1-RR (same clone as 83319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83319-1-RR (SAT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

    IF Staining of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SAT1 antibody (83319-1-RR, Clone: 240157G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • FC experiment of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

    FC experiment of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug SAT1 Recombinant antibody (83319-1-RR, Clone:240157G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00344-2

    Cytometric bead array standard curve of MP00344-2

    Cytometric bead array standard curve of MP00344-2, SAT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83319-3-PBS. Detection antibody: 83319-1-PBS. Standard: Ag1154. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00344-3

    Cytometric bead array standard curve of MP00344-3

    Cytometric bead array standard curve of MP00344-3, SAT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83319-2-PBS. Detection antibody: 83319-1-PBS. Standard:null. Range: null.

  • Affinity and Kinetic Characterization of 83319-1-RR

    Affinity and Kinetic Characterization of 83319-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83319-1-RR against Human SAT1 were performed. The affinity constant is 0.484 nM.

SAT1 Recombinant antibody, PBS Only (Capture/Detector)

SAT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83319-1-PBS

产品说明书

CloneNo. 240157G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1, EC:2.3.1.57

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83319-1-PBS targets SAT1 as part of a matched antibody pair:

MP00344-2: 83319-3-PBS capture and 83319-1-PBS detection (validated in Cytometric bead array)

MP00344-3: 83319-2-PBS capture and 83319-1-PBS detection (validated in Cytometric bead array)

MP00344-4: 83319-1-PBS capture and 83319-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 SAT1 fusion protein Ag1154 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 spermidine/spermine N1-acetyltransferase 1
别名 SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1, EC:2.3.1.57
计算分子量 20 kDa
观测分子量 15-25 kDa
GenBank蛋白编号BC008424
基因名称 SAT1
Gene ID (NCBI) 6303
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21673
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.