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验证数据展示

  • WB analysis using 83319-1-RR

    WB analysis using 83319-1-RR

    Untreated and DENSPM treated HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 83319-1-RR

    WB analysis of K-562 using 83319-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83319-1-RR

    IHC staining of mouse brain using 83319-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83319-1-RR (SAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83319-1-RR

    IHC staining of mouse brain using 83319-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83319-1-RR (SAT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of RAW 264.7 using 83319-1-RR

    IF Staining of RAW 264.7 using 83319-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SAT1 antibody (83319-1-RR, Clone: 240157G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of RAW 264.7 using 83319-1-RR

    FC experiment of RAW 264.7 using 83319-1-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug SAT1 Recombinant antibody (83319-1-RR, Clone:240157G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83319-1-RR

    Affinity and Kinetic Characterization of 83319-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83319-1-RR against Human SAT1 were performed. The affinity constant is 0.484 nM.

SAT1 Recombinant monoclonal antibody

SAT1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83319-1-RR

产品说明书

CloneNo. 240157G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

240157G2, EC:2.3.1.57, Polyamine N-acetyltransferase 1, SAT, Spermidine/spermine N(1)-acetyltransferase 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells
Positive FC (Intra) detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83319-1-RR targets SAT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1154

Product name: Recombinant human SAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-171 aa of BC008424

Sequence: MAKFVIRPATAADCSDILRLIKELAKYEYMEEQVILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSINFYKRRGASDLSSEEGWRLFKIDKEYLLKMATEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 spermidine/spermine N1-acetyltransferase 1
别名 240157G2, EC:2.3.1.57, Polyamine N-acetyltransferase 1, SAT, Spermidine/spermine N(1)-acetyltransferase 1
计算分子量 20 kDa
观测分子量 15-25 kDa
GenBank蛋白编号BC008424
基因名称 SAT1
Gene ID (NCBI) 6303
RRIDAB_3670985
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21673
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.

实验方案

Product Specific Protocols
IF protocol for SAT1 antibody 83319-1-RRDownload protocol
IHC protocol for SAT1 antibody 83319-1-RRDownload protocol
WB protocol for SAT1 antibody 83319-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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