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验证数据展示

  • WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

    WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

    WB analysis using 28625-1-AP (same clone as 28625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 28625-1-AP (same clone as 28625-1-PBS)

    IHC staining of rat brain using 28625-1-AP (same clone as 28625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28625-1-AP (SARM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 28625-1-AP (same clone as 28625-1-PBS)

    IHC staining of rat brain using 28625-1-AP (same clone as 28625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28625-1-AP (SARM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 28625-1-AP (same clone as 28625-1-PBS)

    IF Staining of SH-SY5Y using 28625-1-AP (same clone as 28625-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SARM1 antibody (28625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 28625-1-AP (same clone as 28625-1-PBS)

    FC experiment of SH-SY5Y using 28625-1-AP (same clone as 28625-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SARM1 Polyclonal antibody (28625-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 28625-1-PBS in a different storage buffer formulation.

SARM1 Polyclonal antibody, PBS Only

SARM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 28625-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

EC:3.2.2.-, EC:3.2.2.6, FLJ36296, hSARM1, HsTIR

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28625-1-PBS targets SARM1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29531

Product name: Recombinant human SARM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 352-573 aa of NM_015077

Sequence: EAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTKSALAKRALRLLGEEVPRPILPSVPSWKEAEVQTWLQQIGFSKYCESFREQQVDGDLLLRLTEEELQTDLGMKSGITRKRFFRELTELKTFANYSTCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIHLGVHRARILTAAREMLHSPLPCTGGKPSGDTPDVFISYRRNSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sterile alpha and TIR motif containing 1
别名 EC:3.2.2.-, EC:3.2.2.6, FLJ36296, hSARM1, HsTIR
计算分子量 79 kDa
观测分子量70-80 kDa
GenBank蛋白编号NM_015077
基因名称 SARM1
Gene ID (NCBI) 23098
RRIDAB_3086070
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6SZW1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SARM1 (sterile alpha and Toll/interleukin-1 receptor motif-containing 1) is also named as KIAA0524, SAMD2 and SARM. SARM1 is the major pro-degenerative protein in axons (PMID: 32152523). SARM1 is the central executioner of pathological axon degeneration and is an inducible NAD+-cleavage enzyme that is activated by the loss of the NAD+ biosynthetic enzyme NMNAT2 (PMID: 33657413). SARM1 is involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PMID: 16964262).

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