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SARM1 Polyclonal antibody

SARM1 Polyclonal Antibody for WB,IF/ICC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,IF/ICC,ELISA

Conjugate

Unconjugated

Cat No : 28625-1-AP

Print datasheet

Synonyms

FLJ36296, KIAA0524, SAMD2, SARM, SARM1, Tir 1 homolog

验证数据展示

  • WB analysis using 28625-1-AP

    WB analysis using 28625-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 28625-1-AP

    WB analysis using 28625-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28625-1-AP (SARM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 28625-1-AP

    IF Staining of SH-SY5Y using 28625-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SARM1 antibody (28625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inDaudi cells, HEK-293 cells, Neuro-2a cells, Raji cells, human testis tissue, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28625-1-AP targets SARM1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IF/ICC,ELISA
Tested Reactivity Human, mouse, rat
Immunogen SARM1 fusion protein Ag29531 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterile alpha and TIR motif containing 1
Synonyms FLJ36296, KIAA0524, SAMD2, SARM, SARM1, Tir 1 homolog
Calculated Molecular Weight 79 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberNM_015077
Gene Symbol SARM1
Gene ID (NCBI) 23098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6SZW1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SARM1 (sterile alpha and Toll/interleukin-1 receptor motif-containing 1) is also named as KIAA0524, SAMD2 and SARM. SARM1 is the major pro-degenerative protein in axons (PMID: 32152523). SARM1 is the central executioner of pathological axon degeneration and is an inducible NAD+-cleavage enzyme that is activated by the loss of the NAD+ biosynthetic enzyme NMNAT2 (PMID: 33657413). SARM1 is involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PMID: 16964262).

实验方案

Product Specific Protocols
WB protocol for SARM1 antibody 28625-1-APDownload protocol
IF protocol for SARM1 antibody 28625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols