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CoraLite® Plus 488-conjugated RYR1 Monoclonal antibody

RYR1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B4E12

Cat No : CL488-66539

Print datasheet

Synonyms

CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, SKRR

验证数据展示

  • IF Staining of PC-12 using CL488-66539

    IF Staining of PC-12 using CL488-66539

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 RYR1 antibody (CL488-66539, Clone: 1B4E12 ) at dilution of 1:100.

  • FC experiment of SH-SY5Y using CL488-66539

    FC experiment of SH-SY5Y using CL488-66539

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RYR1 (CL488-66539, Clone:1B4E12) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inPC-12 cells
Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 66539-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66539 targets RYR1 in IF, FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, rat
Immunogen RYR1 fusion protein Ag25120 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ryanodine receptor 1 (skeletal)
Synonyms CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, SKRR
Calculated Molecular Weight 565 kDa
GenBank Accession NumberNM_000540
Gene Symbol RYR1
Gene ID (NCBI) 6261
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21817
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RYR1 antibody CL488-66539Download protocol
FC protocol for CL Plus 488 RYR1 antibody CL488-66539Download protocol
Standard Protocols
Click here to view our Standard Protocols