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验证数据展示

  • IF Staining of PC-12 using CL488-66539

    IF Staining of PC-12 using CL488-66539

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 RYR1 antibody (CL488-66539, Clone: 1B4E12 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated RYR1 Monoclonal antibody

RYR1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66539

产品说明书

CloneNo. 1B4E12

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

IF/ICC

别名

CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, SKRR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 66539-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66539 targets RYR1 in IF/ICC applications and shows reactivity with Human, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, rat
免疫原

CatNo: Ag25120

Product name: Recombinant human RYR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4942-5038 aa of NM_000540

Sequence: GELRDQQEQVKEDMETKCFICGIGSDYFDTTPHGFETHTLEEHNLANYMFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ryanodine receptor 1 (skeletal)
别名 CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, SKRR
计算分子量 565 kDa
GenBank蛋白编号NM_000540
基因名称 RYR1
Gene ID (NCBI) 6261
RRIDAB_3084256
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP21817
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RYR1 antibody CL488-66539Download protocol
FC protocol for CL Plus 488 RYR1 antibody CL488-66539Download protocol
Standard Protocols
Click here to view our Standard Protocols