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验证数据展示

  • IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

    IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

    IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • WB analysis using 12170-1-AP (same clone as 12170-1-PBS)

    WB analysis using 12170-1-AP (same clone as 12170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12170-1-AP (same clone as 12170-1-PBS)

    IP experiment of HeLa using 12170-1-AP (same clone as 12170-1-PBS)

    IP result of anti-RTF1 (IP:12170-1-AP, 4ug; Detection:12170-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

RTF1 Polyclonal antibody, PBS Only

RTF1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12170-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

CDG1N, GTL7, KIAA0252, RNA polymerase-associated protein RTF1 homolog

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12170-1-PBS targets RTF1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2812

Product name: Recombinant human RTF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-307 aa of BC015052

Sequence: MKKQANKTASSGSSDKDSSAESSAPEEGEVSDSDSNSSSSSSDSDSSSEDEEFHDGYGEDLMGDEEDRARLEQMTEKEREQELFNRIEKREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKRDEKLDKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEEDDKSSEKSDRSSRTSSSDEEEEKEEIPPKSQPVSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
别名 CDG1N, GTL7, KIAA0252, RNA polymerase-associated protein RTF1 homolog
计算分子量585aa,67 kDa; 710aa,80 kDa
观测分子量 100 kDa
GenBank蛋白编号BC015052
基因名称 RTF1
Gene ID (NCBI) 23168
RRIDAB_2180931
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92541
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RTF1 (RNA polymerase-associated protein RTF1 homolog) is a multifunctional component of the Paf1 complex that regulates gene expression by directing cotranscriptional histone modification (PMID: 17576814). RTF1 is essential for global methylation of H3-Lys4 and H3-Lys79, but not H3-Lys36 (PMID: 12876293).

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