验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HL-60 cells, rat brain tissue, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IP | See 1 publications below |
产品信息
12170-1-AP targets RTF1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2812 Product name: Recombinant human RTF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-307 aa of BC015052 Sequence: MKKQANKTASSGSSDKDSSAESSAPEEGEVSDSDSNSSSSSSDSDSSSEDEEFHDGYGEDLMGDEEDRARLEQMTEKEREQELFNRIEKREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKRDEKLDKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEEDDKSSEKSDRSSRTSSSDEEEEKEEIPPKSQPVSLPEELNRVRLSRHKLERWCHMPFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) |
| 别名 | CDG1N, GTL7, KIAA0252, RTF1 |
| 计算分子量 | 585aa,67 kDa; 710aa,80 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC015052 |
| 基因名称 | RTF1 |
| Gene ID (NCBI) | 23168 |
| RRID | AB_2180931 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92541 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RTF1 antibody 12170-1-AP | Download protocol |
| IP protocol for RTF1 antibody 12170-1-AP | Download protocol |
| WB protocol for RTF1 antibody 12170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Cell cycle-resolved chromatin proteomics reveals the extent of mitotic preservation of the genomic regulatory landscape. | ||
Cell Death Dis UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer. | ||
Mol Cell ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss | ||
Nat Commun Multiple structures of RNA polymerase II isolated from human nuclei by ChIP-CryoEM analysis |
