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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RRM1 Polyclonal antibody

RRM1 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10526

Print datasheet

Synonyms

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1

验证数据展示

  • FC experiment of A549 using CL488-10526

    FC experiment of A549 using CL488-10526

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RRM1 (CL488-10526) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-10526

    IF Staining of HepG2 using CL488-10526

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 RRM1 antibody (CL488-10526) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10526-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10526 targets RRM1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat, monkey
Immunogen RRM1 fusion protein Ag0789 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonucleotide reductase M1
Synonyms R1, ribonucleotide reductase M1, RIR1, RR1, RRM1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_2918984
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDP23921
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide 

synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair.


What is the molecular weight of RRM1?

The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed 

of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2 

subunits in non-dividing quiescent cells (PMID: 17416930).


What is the subcellular localization of RRM1?

RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2. 

However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953).


What is the tissue expression pattern of RRM1?

RRM1 is ubiquitously expressed.


How is RRM1 expression regulated during the cell cycle and upon DNA damage?

Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase. 

However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to 

RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins 

or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592).


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RRM1 antibody CL488-10526Download protocol
FC protocol for CL Plus 488 RRM1 antibody CL488-10526Download protocol
Standard Protocols
Click here to view our Standard Protocols