- Featured Product
- KD/KO Validated
RRM1 Polyclonal antibody
RRM1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey and More (1)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, A549 cells, K-562 cells, BxPC-3 cells, HEK-293 cells, U-251 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, MCF-7 cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10526-1-AP targets RRM1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse, Xenopus |
| Immunogen | RRM1 fusion protein Ag0789 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ribonucleotide reductase M1 |
| Synonyms | R1, ribonucleotide reductase M1, RIR1, RR1, RRM1 |
| Calculated Molecular Weight | 90 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC006498 |
| Gene Symbol | RRM1 |
| Gene ID (NCBI) | 6240 |
| RRID | AB_2180372 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23921 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide
synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair.
What is the molecular weight of RRM1?
The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed
of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2
subunits in non-dividing quiescent cells (PMID: 17416930).
What is the subcellular localization of RRM1?
RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2.
However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953).
What is the tissue expression pattern of RRM1?
RRM1 is ubiquitously expressed.
How is RRM1 expression regulated during the cell cycle and upon DNA damage?
Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase.
However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to
RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins
or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RRM1 antibody 10526-1-AP | Download protocol |
| IHC protocol for RRM1 antibody 10526-1-AP | Download protocol |
| IF protocol for RRM1 antibody 10526-1-AP | Download protocol |
| IP protocol for RRM1 antibody 10526-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Lancet Oncol The ribonucleotide reductase large subunit (RRM1) as a predictive factor in patients with cancer. | ||
Oncotarget Impact of molecular profiling on overall survival of patients with advanced ovarian cancer. |
