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验证数据展示

  • IF Staining of HepG2 using CL594-66886

    IF Staining of HepG2 using CL594-66886

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66886 (S6 Ribosomal protein antibody) at dilution of 1:100.

CoraLite®594-conjugated S6 Ribosomal protein Monoclonal antibody

S6 Ribosomal protein Monoclonal Antibody for IF/ICC
Cat No. CL594-66886

产品说明书

CloneNo. 1C3E10

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66886-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66886 targets S6 Ribosomal protein in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 S6 Ribosomal protein fusion protein Ag6599 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S6
别名 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6
计算分子量 29 kDa
观测分子量 29-32 kDa
GenBank蛋白编号BC000524
基因名称 RPS6
Gene ID (NCBI) 6194
RRIDAB_2883632
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62753
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

实验方案

Product Specific Protocols
IF protocol for CL594 S6 Ribosomal protein antibody CL594-66886Download protocol
Standard Protocols
Click here to view our Standard Protocols