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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RPS3 Monoclonal antibody

RPS3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G7H4

Cat No : CL488-66046

Print datasheet

Synonyms

40S ribosomal protein S3, FLJ26283, FLJ27450, ribosomal protein S3, Ribosome Marker, RPS3

验证数据展示

  • IF Staining of HepG2 using CL488-66046

    IF Staining of HepG2 using CL488-66046

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66046 (RPS3 antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPS3 Ready-To-Use IHC Kit. RPS3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66046-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-66046 is RPS3 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen RPS3 fusion protein Ag7515 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ribosomal protein S3
Synonyms 40S ribosomal protein S3, FLJ26283, FLJ27450, ribosomal protein S3, Ribosome Marker, RPS3
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC003137
Gene Symbol RPS3
Gene ID (NCBI) 6188
RRIDAB_2883239
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23396
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RPS3 antibody CL488-66046Download protocol
Standard Protocols
Click here to view our Standard Protocols