验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, COLO 320 cells, NIH/3T3 cells, mouse brain tissue, human brain tissue, |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84947-4-RR targets RPS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | RPS3 fusion protein Ag7346 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | ribosomal protein S3 |
别名 | 242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450 |
计算分子量 | 27 kDa |
观测分子量 | 30 kDa |
GenBank蛋白编号 | BC003137 |
基因名称 | RPS3 |
Gene ID (NCBI) | 6188 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P23396 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.
实验方案
Product Specific Protocols | |
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WB protocol for RPS3 antibody 84947-4-RR | Download protocol |
IHC protocol for RPS3 antibody 84947-4-RR | Download protocol |
IF protocol for RPS3 antibody 84947-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |