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验证数据展示

  • WB analysis using 84947-4-RR (same clone as 84947-4-PBS)

    WB analysis using 84947-4-RR (same clone as 84947-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84947-4-RR (RPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84947-4-RR (same clone as 84947-4-PBS)

    IHC staining of human colon cancer using 84947-4-RR (same clone as 84947-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84947-4-RR (RPS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS3 antibody included
  • IF Staining of NIH/3T3 using 84947-4-RR (same clone as 84947-4-PBS)

    IF Staining of NIH/3T3 using 84947-4-RR (same clone as 84947-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RPS3 antibody (84947-4-RR, Clone: 242504F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84947-4-RR

    Affinity and Kinetic Characterization of 84947-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84947-4-RR against Human RPS3 were performed. The affinity constant is below 1 pM.

RPS3 Recombinant monoclonal antibody, PBS Only

RPS3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84947-4-PBS

产品说明书

CloneNo. 242504F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPS3 Ready-To-Use IHC Kit. RPS3 primary antibody included.

产品信息

84947-4-PBS targets RPS3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7346

Product name: Recombinant human RPS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-243 aa of BC003137

Sequence: MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S3
别名 242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450
计算分子量 27 kDa
观测分子量 30 kDa
GenBank蛋白编号BC003137
基因名称 RPS3
Gene ID (NCBI) 6188
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP23396
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

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