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验证数据展示

  • WB analysis using 86270-4-RR

    WB analysis using 86270-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86270-4-RR (RPL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 86270-4-RR

    IF Staining of HepG2 using 86270-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL7A antibody (86270-4-RR, Clone: 250886G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPL7A Recombinant monoclonal antibody

RPL7A Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86270-4-RR

产品说明书

CloneNo. 250886G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

60S ribosomal protein L7a, Large ribosomal subunit protein eL8, PLA X polypeptide, PLA-X polypeptide, ribosomal protein L7a

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, RAW 264.7 cells, mouse kidney tissue, rat kidney tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL7A Ready-To-Use IHC Kit. RPL7A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86270-4-RR targets RPL7A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7543

Product name: Recombinant human RPL7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC005128

Sequence: MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L7a
别名 60S ribosomal protein L7a, Large ribosomal subunit protein eL8, PLA X polypeptide, PLA-X polypeptide, ribosomal protein L7a
计算分子量 266 aa, 30 kDa
观测分子量32 kDa
GenBank蛋白编号BC005128
基因名称 RPL7A
Gene ID (NCBI) 6130
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62424
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].

实验方案

Product Specific Protocols
IF protocol for RPL7A antibody 86270-4-RRDownload protocol
WB protocol for RPL7A antibody 86270-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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