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验证数据展示

  • WB analysis using 86270-4-RR (same clone as 86270-4-PBS)

    WB analysis using 86270-4-RR (same clone as 86270-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86270-4-RR (RPL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86270-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86270-4-RR (same clone as 86270-4-PBS)

    IF Staining of HepG2 using 86270-4-RR (same clone as 86270-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL7A antibody (86270-4-RR, Clone: 250886G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86270-4-PBS in a different storage buffer formulation.

RPL7A Recombinant monoclonal antibody, PBS Only

RPL7A Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86270-4-PBS

产品说明书

CloneNo. 250886G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

60S ribosomal protein L7a, Large ribosomal subunit protein eL8, PLA X polypeptide, PLA-X polypeptide, ribosomal protein L7a

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPL7A Ready-To-Use IHC Kit. RPL7A primary antibody included.

产品信息

86270-4-PBS targets RPL7A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7543

Product name: Recombinant human RPL7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC005128

Sequence: MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L7a
别名 60S ribosomal protein L7a, Large ribosomal subunit protein eL8, PLA X polypeptide, PLA-X polypeptide, ribosomal protein L7a
计算分子量 266 aa, 30 kDa
观测分子量32 kDa
GenBank蛋白编号BC005128
基因名称 RPL7A
Gene ID (NCBI) 6130
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62424
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].

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