验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse eye tissue |
For other applications, we recommend the unconjugated version of this antibody, 17939-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-17939 targets RPE65 in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RPE65 fusion protein Ag12356 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinal pigment epithelium-specific protein 65kDa |
| 别名 | LCA2, mRPE65, rd12, Retinoid isomerohydrolase, Retinol isomerase, RP20, RPE65, sRPE65 |
| 计算分子量 | 61 kDa |
| 观测分子量 | 65 kDa, 61 kDa |
| GenBank蛋白编号 | BC075036 |
| 基因名称 | RPE65 |
| Gene ID (NCBI) | 6121 |
| RRID | AB_3084013 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16518 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RPE65 antibody CL488-17939 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |