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验证数据展示

  • IF Staining of A431 using CL555-67999

    IF Staining of A431 using CL555-67999

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®555 RPA2 antibody (CL555-67999, Clone: 1B9H11 ) at dilution of 1:1000.

  • FC experiment of HeLa using CL555-67999

    FC experiment of HeLa using CL555-67999

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human RPA2 (CL555-67999, Clone:1B9H11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®555-conjugated RPA2 Monoclonal antibody

RPA2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-67999

产品说明书

CloneNo. 1B9H11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®555
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67999-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67999 targets RPA2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag24783

Product name: Recombinant human RPA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 80-270 aa of BC001630

Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 replication protein A2, 32kDa
别名 RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2
计算分子量 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC001630
基因名称 RPA2
Gene ID (NCBI) 6118
RRIDAB_2934688
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP15927
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.

实验方案

Product Specific Protocols
IF protocol for CL555 RPA2 antibody CL555-67999Download protocol
Standard Protocols
Click here to view our Standard Protocols
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