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验证数据展示

  • WB analysis using 67999-1-Ig

    WB analysis using 67999-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of A431 using 67999-1-Ig

    IF Staining of A431 using 67999-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

RPA2 Monoclonal antibody

RPA2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67999-1-Ig

产品说明书

CloneNo. 1B9H11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, pig spleen tissue, rat testis tissue, mouse testis tissue, rabbit spleen tissue, Hela cells, MOLT-4 cells, Jurkat cells, THP-1 cells
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67999-1-Ig targets RPA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag24783

Product name: Recombinant human RPA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 80-270 aa of BC001630

Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 replication protein A2, 32kDa
别名 RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2
计算分子量 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC001630
基因名称 RPA2
Gene ID (NCBI) 6118
RRIDAB_2918747
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP15927
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.

实验方案

Product Specific Protocols
IF protocol for RPA2 antibody 67999-1-IgDownload protocol
WB protocol for RPA2 antibody 67999-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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